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rabbit polyclonal antibody against h3t11p  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit polyclonal antibody against h3t11p
    Analysis of epigenetic histone modifications during mitosis and micronuclei formation. Whole-mount immunofluorescent staining of gonads from sexual species ( a , b ) and hybrids (all rest) with antibodies against various epigenetic modification stained in red: H3S10P ( a , c , d ), H3S28P ( b , e ), H3K9me3 ( f , g ) and <t>H3T11P</t> ( h ). Accumulation of all analyzed chromatin modifications indicate normal chromosome condensation during prophase and metaphase. In hybrids, misaligned chromosomes (indicated by white arrows) have similar distribution of the H3S10P ( c ), H3S28P ( e ), H3K9me3 ( f ) and H3T11P ( h ) chromatin modifications as the rest of the chromosomes (showed by white arrowheads) suggesting that misaligned chromosomes condense properly during prophase and metaphase. Micronuclei present during metaphases (indicated by thin white arrows) do not accumulate epigenetic histone modification such as H3S10P ( c ), H3K9me3 ( f ). ( d ) Accumulation of H3S10P epigenetic modification in some micronuclei (indicated by thin red arrows) suggest their formation by chromosomal lagging. Some micronuclei do not show accumulation of H3S10P epigenetic modification (indicated by thin white arrows). ( g ) In contrast to the chromatin in the interphase nucleus of the gonial cell, chromatin in micronuclei accumulated H3K9me3 epigenetic modifications indicating its inactivation and heterochromatinization (micronuclei indicated with thin red arrows). Tubulin (stained in green) visualize cytoskeleton components of the spindle ( a , f ). Chromatin is visualized with DAPI in blue. All pictures represent a single gonadal section of 0.4 μm in thickness after 3D immunofluorescent staining. Scale bars = 10 μm.
    Rabbit Polyclonal Antibody Against H3t11p, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal antibody against h3t11p/product/Cell Signaling Technology Inc
    Average 93 stars, based on 27 article reviews
    rabbit polyclonal antibody against h3t11p - by Bioz Stars, 2026-02
    93/100 stars

    Images

    1) Product Images from "Gradual chromosomal lagging drive programmed genome elimination in hemiclonal fishes from the genus Hypseleotris"

    Article Title: Gradual chromosomal lagging drive programmed genome elimination in hemiclonal fishes from the genus Hypseleotris

    Journal: Scientific Reports

    doi: 10.1038/s41598-024-78278-6

    Analysis of epigenetic histone modifications during mitosis and micronuclei formation. Whole-mount immunofluorescent staining of gonads from sexual species ( a , b ) and hybrids (all rest) with antibodies against various epigenetic modification stained in red: H3S10P ( a , c , d ), H3S28P ( b , e ), H3K9me3 ( f , g ) and H3T11P ( h ). Accumulation of all analyzed chromatin modifications indicate normal chromosome condensation during prophase and metaphase. In hybrids, misaligned chromosomes (indicated by white arrows) have similar distribution of the H3S10P ( c ), H3S28P ( e ), H3K9me3 ( f ) and H3T11P ( h ) chromatin modifications as the rest of the chromosomes (showed by white arrowheads) suggesting that misaligned chromosomes condense properly during prophase and metaphase. Micronuclei present during metaphases (indicated by thin white arrows) do not accumulate epigenetic histone modification such as H3S10P ( c ), H3K9me3 ( f ). ( d ) Accumulation of H3S10P epigenetic modification in some micronuclei (indicated by thin red arrows) suggest their formation by chromosomal lagging. Some micronuclei do not show accumulation of H3S10P epigenetic modification (indicated by thin white arrows). ( g ) In contrast to the chromatin in the interphase nucleus of the gonial cell, chromatin in micronuclei accumulated H3K9me3 epigenetic modifications indicating its inactivation and heterochromatinization (micronuclei indicated with thin red arrows). Tubulin (stained in green) visualize cytoskeleton components of the spindle ( a , f ). Chromatin is visualized with DAPI in blue. All pictures represent a single gonadal section of 0.4 μm in thickness after 3D immunofluorescent staining. Scale bars = 10 μm.
    Figure Legend Snippet: Analysis of epigenetic histone modifications during mitosis and micronuclei formation. Whole-mount immunofluorescent staining of gonads from sexual species ( a , b ) and hybrids (all rest) with antibodies against various epigenetic modification stained in red: H3S10P ( a , c , d ), H3S28P ( b , e ), H3K9me3 ( f , g ) and H3T11P ( h ). Accumulation of all analyzed chromatin modifications indicate normal chromosome condensation during prophase and metaphase. In hybrids, misaligned chromosomes (indicated by white arrows) have similar distribution of the H3S10P ( c ), H3S28P ( e ), H3K9me3 ( f ) and H3T11P ( h ) chromatin modifications as the rest of the chromosomes (showed by white arrowheads) suggesting that misaligned chromosomes condense properly during prophase and metaphase. Micronuclei present during metaphases (indicated by thin white arrows) do not accumulate epigenetic histone modification such as H3S10P ( c ), H3K9me3 ( f ). ( d ) Accumulation of H3S10P epigenetic modification in some micronuclei (indicated by thin red arrows) suggest their formation by chromosomal lagging. Some micronuclei do not show accumulation of H3S10P epigenetic modification (indicated by thin white arrows). ( g ) In contrast to the chromatin in the interphase nucleus of the gonial cell, chromatin in micronuclei accumulated H3K9me3 epigenetic modifications indicating its inactivation and heterochromatinization (micronuclei indicated with thin red arrows). Tubulin (stained in green) visualize cytoskeleton components of the spindle ( a , f ). Chromatin is visualized with DAPI in blue. All pictures represent a single gonadal section of 0.4 μm in thickness after 3D immunofluorescent staining. Scale bars = 10 μm.

    Techniques Used: Staining, Modification



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    rabbit polyclonal antibody against h3t11p  (Cell Signaling Technology Inc)
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    Cell Signaling Technology Inc rabbit polyclonal antibody against h3t11p
    Analysis of epigenetic histone modifications during mitosis and micronuclei formation. Whole-mount immunofluorescent staining of gonads from sexual species ( a , b ) and hybrids (all rest) with antibodies against various epigenetic modification stained in red: H3S10P ( a , c , d ), H3S28P ( b , e ), H3K9me3 ( f , g ) and <t>H3T11P</t> ( h ). Accumulation of all analyzed chromatin modifications indicate normal chromosome condensation during prophase and metaphase. In hybrids, misaligned chromosomes (indicated by white arrows) have similar distribution of the H3S10P ( c ), H3S28P ( e ), H3K9me3 ( f ) and H3T11P ( h ) chromatin modifications as the rest of the chromosomes (showed by white arrowheads) suggesting that misaligned chromosomes condense properly during prophase and metaphase. Micronuclei present during metaphases (indicated by thin white arrows) do not accumulate epigenetic histone modification such as H3S10P ( c ), H3K9me3 ( f ). ( d ) Accumulation of H3S10P epigenetic modification in some micronuclei (indicated by thin red arrows) suggest their formation by chromosomal lagging. Some micronuclei do not show accumulation of H3S10P epigenetic modification (indicated by thin white arrows). ( g ) In contrast to the chromatin in the interphase nucleus of the gonial cell, chromatin in micronuclei accumulated H3K9me3 epigenetic modifications indicating its inactivation and heterochromatinization (micronuclei indicated with thin red arrows). Tubulin (stained in green) visualize cytoskeleton components of the spindle ( a , f ). Chromatin is visualized with DAPI in blue. All pictures represent a single gonadal section of 0.4 μm in thickness after 3D immunofluorescent staining. Scale bars = 10 μm.
    Rabbit Polyclonal Antibody Against H3t11p, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal antibody against h3t11p/product/Cell Signaling Technology Inc
    Average 93 stars, based on 1 article reviews
    rabbit polyclonal antibody against h3t11p - by Bioz Stars, 2026-02
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Analysis of epigenetic histone modifications during mitosis and micronuclei formation. Whole-mount immunofluorescent staining of gonads from sexual species ( a , b ) and hybrids (all rest) with antibodies against various epigenetic modification stained in red: H3S10P ( a , c , d ), H3S28P ( b , e ), H3K9me3 ( f , g ) and H3T11P ( h ). Accumulation of all analyzed chromatin modifications indicate normal chromosome condensation during prophase and metaphase. In hybrids, misaligned chromosomes (indicated by white arrows) have similar distribution of the H3S10P ( c ), H3S28P ( e ), H3K9me3 ( f ) and H3T11P ( h ) chromatin modifications as the rest of the chromosomes (showed by white arrowheads) suggesting that misaligned chromosomes condense properly during prophase and metaphase. Micronuclei present during metaphases (indicated by thin white arrows) do not accumulate epigenetic histone modification such as H3S10P ( c ), H3K9me3 ( f ). ( d ) Accumulation of H3S10P epigenetic modification in some micronuclei (indicated by thin red arrows) suggest their formation by chromosomal lagging. Some micronuclei do not show accumulation of H3S10P epigenetic modification (indicated by thin white arrows). ( g ) In contrast to the chromatin in the interphase nucleus of the gonial cell, chromatin in micronuclei accumulated H3K9me3 epigenetic modifications indicating its inactivation and heterochromatinization (micronuclei indicated with thin red arrows). Tubulin (stained in green) visualize cytoskeleton components of the spindle ( a , f ). Chromatin is visualized with DAPI in blue. All pictures represent a single gonadal section of 0.4 μm in thickness after 3D immunofluorescent staining. Scale bars = 10 μm.

    Journal: Scientific Reports

    Article Title: Gradual chromosomal lagging drive programmed genome elimination in hemiclonal fishes from the genus Hypseleotris

    doi: 10.1038/s41598-024-78278-6

    Figure Lengend Snippet: Analysis of epigenetic histone modifications during mitosis and micronuclei formation. Whole-mount immunofluorescent staining of gonads from sexual species ( a , b ) and hybrids (all rest) with antibodies against various epigenetic modification stained in red: H3S10P ( a , c , d ), H3S28P ( b , e ), H3K9me3 ( f , g ) and H3T11P ( h ). Accumulation of all analyzed chromatin modifications indicate normal chromosome condensation during prophase and metaphase. In hybrids, misaligned chromosomes (indicated by white arrows) have similar distribution of the H3S10P ( c ), H3S28P ( e ), H3K9me3 ( f ) and H3T11P ( h ) chromatin modifications as the rest of the chromosomes (showed by white arrowheads) suggesting that misaligned chromosomes condense properly during prophase and metaphase. Micronuclei present during metaphases (indicated by thin white arrows) do not accumulate epigenetic histone modification such as H3S10P ( c ), H3K9me3 ( f ). ( d ) Accumulation of H3S10P epigenetic modification in some micronuclei (indicated by thin red arrows) suggest their formation by chromosomal lagging. Some micronuclei do not show accumulation of H3S10P epigenetic modification (indicated by thin white arrows). ( g ) In contrast to the chromatin in the interphase nucleus of the gonial cell, chromatin in micronuclei accumulated H3K9me3 epigenetic modifications indicating its inactivation and heterochromatinization (micronuclei indicated with thin red arrows). Tubulin (stained in green) visualize cytoskeleton components of the spindle ( a , f ). Chromatin is visualized with DAPI in blue. All pictures represent a single gonadal section of 0.4 μm in thickness after 3D immunofluorescent staining. Scale bars = 10 μm.

    Article Snippet: We used the following primary antibodies: rabbit polyclonal antibody against DDX4 (concentration 1:50; C1C3, GeneTex) to detect Vasa protein; mouse monoclonal antibodies against alpha tubulin (concentration 1:100; ab7291, Abcam); rabbit polyclonal antibody against H3S10P (concentration 1:100; GTX128116, GeneTex); rabbit polyclonal antibody against H3S28P (concentration 1:100; #9713, Cell Signaling); rabbit polyclonal antibody against H3T11P (concentration 1:100; #9764, Cell Signaling); rabbit polyclonal antibody against H3K9me3 (concentration 1:100; ab8898, Abcam).

    Techniques: Staining, Modification